07-1383

Anti-CDT1 Antibody

serum, from rabbit

• 동의어(들): DNA replication factor , Double parked homolog , Double parked, Drosophila, homolog of 2 chromatin licensing and DNA replication factor 1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: serum
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 종 반응성: human, mouse, rat
• 기술: immunoprecipitation (IP): suitable; western blot: suitable
• NCBI 수납 번호: NP_112190.2
• UniProt 수납 번호: Q9H211
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... CDT1(81620) ; mouse ... Cdt1(67177) ; rat ... Cdt1(292071)

설명

일반 설명

CDT1 (Chromatin Licensing and DNA replication Factor 1) is an essential component of the cell cycle machinery. It plays an important role in insuring that there is only one round of DNA replication per cell cycle. CDT1 is a known substrate of Cdk2 and Cdk4 (Liu, E. 2004). It is presented in G1 of the cell cycle and is degraded as cells enter S phase (Liu, E. 2004). In higher eukaryotes, CDT1 is known to interact with Geminin, a protein that is degraded during mitosis, that inhibits it. CDT1 is marked for degradation by two E3 ligases: SCF complex and the CUL4-DDB1 complex (Sansam, 2006).

특이성

This antibody recognizes the Cdt1.

면역원

Epitope: C-terminal half

Recombinant protein including amino acids 238-546 of human CDT1.

애플리케이션

An independent lab detected this antibody to immunoprecipitate (IP) Cdt1 using 2 μL/IP reaction (Wohlschlegel, B.T., 2000).

Detect CDT1 using this Anti-CDT1 Antibody validated for use in WB & IP.

Research Category
Signaling

Research Sub Category
Cell Cycle, DNA Replication & Repair

품질

Evaluated by western blot on Jurkat cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody was used to detect CDT1 in Jurkat cell lysate.

표적 설명

65 kDa

물리적 형태

Unpurified rabbit serum containing 0.05% NaN3.

저장 및 안정성

Stable for 2 years at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1