07-1285

Anti-acetylated-Src (Lys430) Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): SRC
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human
• 종 반응성(상동성에 의해 예측): mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
• 기술: cell based assay: suitable; western blot: suitable
• NCBI 수납 번호: NP_005408
• UniProt 수납 번호: P12931
• 배송 상태: wet ice
• 타겟 번역 후 변형: acetylation (Lys430)
• 유전자 정보: human ... SRC(6714)

설명

일반 설명

Src is a proto-oncogenic tyrosine kinase belonging to the Src family of non-receptor tyrosine kinases. This proto-oncogene plays a pivotal role cellular processes such as proliferation, migration, transformation and regulation of embyronic development and cell growth. Src tyrosine kinases transmit integrin-dependent signals for cell movement and proliferation. The study of Src proteins have contributed to the understanding of cancer caused by abnormal cellular signaling.

특이성

This antibody recognizes Src acetylated at Lys430.

면역원

Epitope: Acetylated Lys430

KLH-conjugated linear peptide corresponding to human Src acetylated at Lys430.

애플리케이션

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

This Anti-acetylated-Src (Lys430) Antibody is validated for use in WB, Cell Function Assay for the detection of acetylated-Src (Lys430).

Western Blot Analysis: 5 µg/mL from a representative lot detected Src on 10 µg of HEK293T cells transfected with myc-tagged Src and CBP.

품질

Western Blot/Peptide Inhibition Assay Analysis: This antibody peptide blocked on HEK293T cells transfected with myc-tagged Src and CBP.

표적 설명

~69 kDa observed.
Endogenous Src has a molecular weight of ~60 kDa; however, the myc tag adds ~9 kDa to the weight of the protein.

물리적 형태

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
HEK293T cells transfected with myc-tagged Src and CBP

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids