07-1216

Anti-PP2A Antibody, Catalytic subunit

Upstate^®, from rabbit

• 동의어(들): Serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, delta isoform, Delta isoform of regulatory subunit B56, protein phosphatase, Protein phosphatase 2, regulatory subunit B (B56), delta isoform, Protein phosphatase 2, regulatory subunit B
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: mouse, chicken, bovine, rat, human
• 제조업체/상표: Upstate^®
• 기술: immunohistochemistry: suitable (paraffin); western blot: suitable
• 동형: IgG
• NCBI 수납 번호: NM_006245
• UniProt 수납 번호: Q14738
• 배송 상태: dry ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... PPP2R5D(5528)

설명

일반 설명

PP2A is a type 2 protein Ser/Thr phosphatase characterized by high activity against the α subunit of Phosphorylase Kinase and low sensitivity to Inhibitor-2. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and its high sensitivity to Okadaic Acid. It is also thought to be the principal downmodulator of Ser/Thr kinase cascades. PP2A is a heterotrimer containing a structural subunit (A), a regulatory subunit (B), and a catalytic subunit (C). There are multiple isotypes (encoded by distinct genes) of the B subunit, grouped into three families: B/PR55, B′/PR61, and B′′/PR72 . PTPA is a member of the B′ family that is required for activation of PP2A by an undefined mechanism.

특이성

Peptide is highly conserved in human, rat, bovine, mouse and chicken.

Recognizes protein phosphatase 2A, catalytic subunit.

면역원

Epitope: C-terminus

Synthetic peptide corresponding to a.a. 298-309 of the human sequence.

애플리케이션

Immunohistochemisty (paraffin): Antibody was diluted to 1:100, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen as staining in normal human testis.

Research Category
Signaling

Research Sub Category
Kinases & Phosphatases

This Anti-PP2A Antibody, Catalytic subunit is validated for use in WB, IH(P) for the detection of PP2A.

품질

Routinely evaluated by Western Blot on HEK293 lysates.
Western Blot Analysis:
1:500-1:1,000 dilution is traditonally used to detected PP2A, Catalytic subunit in 10 μg of HEK293 lysates.

표적 설명

60 kDa

결합

Replaces: AB1621

물리적 형태

Affinity purified

Purified rabbit polyclonal IgG in buffer containing PBS with no preservatives.

저장 및 안정성

Stable for 1 year at -80°C from date of receipt. Avoid repeated freeze/thaw.

분석 메모

Control
HEK293 cell lysate

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable