06-868

Anti-GABAA Receptor α1 Antibody

Upstate^®, from rabbit

• 동의어(들): Gamma-amino butyric acid receptor subunit alpha-1, GABA(A) receptor subunit alpha-1, gamma-aminobutyric acid (GABA) A receptor, alpha 1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: mouse, rat
• 제조업체/상표: Upstate^®
• 기술: immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• NCBI 수납 번호: Np_899155
• UniProt 수납 번호: P62813
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: mouse ... Gabra1(14394) ; rat ... Gabra1(29705)

설명

일반 설명

The neurotransmitter GABA (gamma-aminobutyric acid) binds to specific transmembrane receptors in the plasma membrane of both pre- and postsynaptic neurons. Three general classes of GABA receptors are known: GABAA and GABAC (ionotropic) receptors and GABAB (metabotropic) receptors. The GABAA (gamma-Aminobutyric Acid type A) receptor is a multiprotein complex that acts as a ligand-gated chloride channel. These receptors are hetero-oligomers, for which 6 subunits are currently known (a, b, g, d, e and r). There are at least 6 different isoforms of the alpha subunit, and receptor subunit expression varies during development. Increased alpha1 subunit assembly in postsynaptic receptors of cerebellar inhibitory synapses is responsible for the fast inhibitory synaptic currents observed during postnatal development. Additionally, alpha1-containing GABAA receptors are important in regulating the reinforcing properties of alcohol, and expression of the alpha1 subunit increases upon chronic exposure to alcohol, making it a potential therapeutic target for alcohol dependency.

특이성

Other homologies: Human (94% sequence homology).

This antibody recognizes the topographical domain of GABAA Receptor α1.

면역원

Epitope: Topological domain.

KLH-conjugated linear peptide corresponding to the topological domain of rat GABAA Receptor α1.

애플리케이션

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected GABAA Receptor α1 in rat hippocampus tissue.

Immunoprecipitation Analysis: 5 µL from a representative lot immunoprecipitated GABAA Receptor α1 in rat brain microsomal preparation RIPA lysate.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

This Anti-GABAA Receptor α1 Antibody is validated for use in IH, IP, WB for the detection of GABAA Receptor α1.

품질

Evaluated by Western Blot in rat brain microsomal preparation tissue lysate.

Western Blot Analysis: A 1:10,000 dilution of this antibody detected GABAA Receptor α1 in 10 µg of rat brain microsomal preparation tissue lysate.

표적 설명

~51 kDa observed

물리적 형태

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
Rat Brain Microsomal Preparation Tissue Lysate.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1