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Merck
모든 사진(1)

주요 문서

06-418

Sigma-Aldrich

Anti-NFκB p65 Antibody, CT

Upstate®, from rabbit

동의어(들):

Nuclear factor NF-kappa-B p65 subunit, v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear

factor of kappa light polypeptide gene enhancer in B-cells 3 (p65)), v-rel reticuloendotheliosis viral oncogene homolog A (avian), v-rel r

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

rabbit

Quality Level

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

polyclonal

종 반응성

mouse, rat, human

제조업체/상표

Upstate®

기술

electrophoretic mobility shift assay: suitable
immunoprecipitation (IP): suitable
western blot: suitable

동형

IgG

NCBI 수납 번호

UniProt 수납 번호

배송 상태

dry ice
wet ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... NFKB1(4790)

일반 설명

The transcription factor NFκB (Nuclear Factor κB) is involved in the expression and regulation of a number of important cellular and physiological processes such as growth, development, apoptosis, immune and inflammatory response, and activation of various viral promoters including human immunodeficiency virus long terminal repeats. NFκB represents a group of structurally related and evolutionarily conserved proteins related to the proto-oncogene c-Rel with five members in mammals that include Rel (cRel), RelA (p65), RelB, NFκB1 (p50 and its precursor p105), and NFκB2 (p52 and its precursor p100). NFκB/Rel proteins exist as homo- or heterodimers to form transcriptionally competent or repressive complexes. Although most NFκB dimers are activators of transcription, the p50/50 and p52/52 homodimers can repress the transcription of their target genes. The p50/p65 heterodimer of NFκB is the most abundant in cells.

특이성

NFκB p65, Mr 65 kDa

면역원

Epitope: C-terminus
KLH-conjugated, synthetic peptide corresponding to the 13 C-terminal amino acids (C-DMDFSALLSQISS) of the p65 subunit of human NFκB containing an N-terminal cysteine for conjugation. The immunizing sequence is identical in mouse.

애플리케이션

Anti-NFκB p65 Antibody, CT is a Rabbit Polyclonal Antibody for detection of NFκB p65 also known as Nuclear factor NF-kappa-B p65 subunit & has been validated in EMSA, IP & WB.
Immunoprecipitation:
4 µg of a previous lot immunoprecipitated NFκB p65 from 500 μg of a human A431 carcinoma cell lysate.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

품질

Routinely evaluated by Western Blot on RIPA lysates from A431 cells.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected NFkB p65 in RIPA lysates from A431 cells.

표적 설명

65 kDa

결합

Replaces: 04-1008; 04-235

물리적 형태

Format: Purified
Protein A chromatography
Purified rabbit polyclonal IgG in 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol. Liquid at -20°C.

저장 및 안정성

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.

분석 메모

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1


시험 성적서(COA)

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문서

The term neurodegeneration characterizes a chronic loss of neuronal structure and function leading to progressive mental impairments.

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