06-1354

Anti-mRNA-capping enzyme Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): mRNA-capping enzyme, HCAP1, HCE, Polynucleotide 5′-triphosphatase, mRNA 5′-triphosphatase, Tpase, mRNA guanylyltransferase, GTP--RNA guanylyltransferase, GTase
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: mouse, human
• 기술: immunocytochemistry: suitable; western blot: suitable
• NCBI 수납 번호: NP_003791
• UniProt 수납 번호: O60942

설명

일반 설명

mRNA capping enzyme is one of the first mRNA transcription cycle factors to be associated with the C-terminal domain (CTD) and functions by adding an inverted guanylyl group (also known as a ‘cap’) to the 5’ end. mRNA capping enzyme also has triphosphatase and guanylyltransferase activity. The CTD also serves to regulate the capping enzyme and has the ability to remove the Ser5 phosphate which is linked to the release of the capping enzyme from the capping complex. mRNA capping enzyme has a variety of other functions during transcription including; stabilization of polymerase II, activation of early elongation and reduction of re-initiation.

면역원

GST-tagged recombinant protein corresponding to human mRNA-capping enzyme.

애플리케이션

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected mRNA-capping enzyme in NIH/3T3, A431, and HeLa cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Use Anti-mRNA-capping enzyme Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect mRNA-capping enzyme also known as mRNA-capping enzyme, HCAP1, HCE.

품질

Evaluated by Western Blot in HeLa nuclear extract.

Western Blot Analysis: 1:5,000 dilution of this antibody detected mRNA-capping enzyme on 10 µg of HeLa nuclear extract.

표적 설명

~66 kDa observed

물리적 형태

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
HeLa nuclear extract

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable