06-1063

Anti-Frizzled-7 Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): frizzled homolog 7, Frizzled, homolog of, 7, frizzled-7, frizzled homolog 7
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: rat, human, mouse
• 기술: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• NCBI 수납 번호: NP_003498.1
• UniProt 수납 번호: O75084
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... FZD7(8324)

설명

일반 설명

Frizzled 7 is a multi-pass membrane protein belonging to the G-protein coupled receptor Fz/Smo family. It is a receptor for Wnt signaling, predominantly Wnt11, a crucial factor in the regulation of cellular migration and polarization during vertebrate gastrulation. Highest reported expression is in fetal kidney tissue and adult heart tissue. Lower levels have been observed in brain, placenta, adult heart, fetal lung, and squamous cell esophageal carcinomas.

특이성

Recognizes Frizzled-7

면역원

Epitope: Unknown

KLH-conjugated linear peptide corresponding to human Frizzled-7.

애플리케이션

Research Category
Signaling

Research Sub Category
Developmental Signaling

This Anti-Frizzled-7 Antibody is validated for use in WB, IH, IC for the detection of Frizzled-7.

Western Blot Analysis: A previous lot detected Frizzled-7 on 10 µg of HEK293 cell lysate and rat brain microsomal fraction tissue lysate.

Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected Frizzled-7 in human ductal in situ and GI tract tissue.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Frizzled-7 in A431 cells.

품질

Evaluated by Western Blot in mouse brain microsomal fraction tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Frizzled-7 on 10 µg of mouse brain microsomal fraction tissue lysate.

표적 설명

The calculated molecular weight is 64 kDa Observed at ~83 kDa, most likely due to multiple glycosolation sites.

물리적 형태

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
Mouse brain microsomal fraction tissue lysate

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable