05-829-AF555

Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor^™ 555 Conjugate

clone DM1A, from mouse, ALEXA FLUOR^™ 555

• 동의어(들): Tubulin alpha-1 chain, Alpha-tubulin, Alpha-1 tubulin
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 결합: ALEXA FLUOR^™ 555
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: DM1A, monoclonal
• 종 반응성: guinea pig, gerbil, mouse, bovine, avian, human, rat, porcine
• 기술: immunocytochemistry: suitable
• 동형: IgG1
• NCBI 수납 번호: NP_001292201.1
• UniProt 수납 번호: P02552
• 타겟 번역 후 변형: unmodified

설명

일반 설명

Tubulin alpha-1 chain (UniProt P02552) is encoded by the TUBA1C (also known as TUBA1) gene (Gene ID 429035) in Gallus gallus (Chicken) species. Microtubules are cytoskeletal filaments composed of of alpha-/beta-tubulin heterodimers that self-assemble head-to-tail to form protofilaments and laterally to form a hollow tubes. Various post-translational modifications (PTMs), including acetylation, polyglutamylation, polyglycylation, detyrosination, phosphorylation, and palmitoylation, can regulate the polymerization properties of tubulins and/or their interactions with microtubule associated proteins (MAPs) and motor proteins.

특이성

Clone DM1A recognizes α-Tubulin, MW 50-60 kDa. The epitope recognized by this antibody has been mapped to the C-terminal 25 amino acids (AALEKDYEEVGVDSVEGEGEEEGEE).

면역원

Epitope: C-terminal 25 amino acids.

Purified chicken brain α-tubulin.

애플리케이션

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

The unconjugated antibody (Cat. No. 05-829) is shown to be suitable also for immunocytochemistry, immunofluorescence, and Western blotting applications.

This Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor^™ 555 Conjugate is validated for use in Immunocytochemistry for the detection of α-Tubulin.

품질

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected α-tubulin in A431 cells.

표적 설명

~50 kDa observed.

물리적 형태

Protein G purified.

Purified mouse monoclonal IgG1 antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

법적 정보

ALEXA FLUOR is a trademark of Life Technologies

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable