05-354

Anti-Clusterin α chain (human) Antibody, clone 41D

clone 41D, Upstate^®, from mouse

• 동의어(들): Apolipoprotein J, Complement cytolysis inhibitor, Complement-associated protein SP-40,40, Ku70-binding protein 1, Testosterone-repressed prostate message 2, aging-associated protein 4, clusterin, complement lysis inhibitor, complement-associated protein
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• 클론: 41D, monoclonal
• application: ICC; IHC (p); WB
• 종 반응성: human
• 기술: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• citations: 28

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 41D, monoclonal
• 종 반응성: human
• 제조업체/상표: Upstate^®
• 기술: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NM_203339
• UniProt 수납 번호: P10909
• 배송 상태: dry ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... CLU(1191)

설명

일반 설명

Clusterin is also known as apolipoprotein J, complement lysis inhibitor, gp80, glycoprotein III, SGP-2, SP 40-40, TRPM2 and T64. It is a ubiquitous multifunctional protein of 80kD comprising of two disulfide-linked subunits, alpha and beta, and capable of interacting with a broad range of molecules. It is implicated in numerous biological processes including sperm maturation, lipid transport, regulation of the complement cascade, membrane recycling, cell death, immune regulation, cell adhesion and morphological transformation. In pathological conditions, it is an amyloid associated protein co-localising with fibrillar deposits in systemic and localised amyloid disorders. In Alzheimer ’s disease, clusterin is present in amyloid plaques and cerebrovascular deposits. In breast cancers, clusterin expression was found to correlate with tumour size. Clusterin is also implicated with endometrial cancers, myocardial infarction, renal diseases and atherosclerosis.

특이성

This antibody recognizes human clusterin α chain, Mr 40 kDa, and precursor protein.

면역원

Native clusterin purified from human serum. Clone 41D.

애플리케이션

Anti-Clusterin α chain (human) Antibody, clone 41D is an antibody against Clusterin α chain (human) for use in IC, IH, IH(P) & WB.

Immunohistochemistry:
Previous lots of this antibody at 5 µg/mL detected clusterin in paraffin embedded human brain sections.

Research Category
Apoptosis & Cancer

Neuroscience

Research Sub Category
Apoptosis - Additional

Neurodegenerative Diseases

품질

Routinely evaluated on RIPA lysates from HeLa cells or MCF-7 cell lysates.

Western Blot Analysis:
0.5-2 µg/mL of a previous lot detected human clusterin α chain in RIPA lysates from HeLa cells; previous lots detected the protein in human serum and MCF-7 cell lysates

표적 설명

40 kDa

물리적 형태

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, with 0.05% sodium azide. Frozen at -20°C.

저장 및 안정성

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

분석 메모

Control
HeLa cell lysate.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable