05-338

Anti-Fas Antibody (human, neutralizing), clone ZB4

clone ZB4, Upstate^®, from mouse

• 동의어(들): APO-1 cell surface antigen, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, CD95 antigen, FASLG receptor, Fas (TNF receptor superfamily, member 6), Fas AMA, Fas antigen, apoptosis antigen 1, tumor necrosis factor receptor superfamily member 6, tu
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: ZB4, monoclonal
• 종 반응성: human
• 제조업체/상표: Upstate^®
• 기술: flow cytometry: suitable; neutralization: suitable; western blot: suitable
• 동형: IgG1
• NCBI 수납 번호: NM_152877
• UniProt 수납 번호: P25445
• 배송 상태: dry ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... FAS(355)

설명

일반 설명

Fas (also known as Apo-1 and CD95) is a 40-50 kDa cell surface glycoprotein and a member of the TNF receptor family. Fas is an apotosis signalling molecule that forms the death inducing signalling complex (DISC) upon binding by the Fas ligand trimer on an adjacent cell. The Fas receptor also trimerizes, after which it is internalized and can then bind to the adaptor molecule FADD. FADD facilitates binding of caspase-8. which is activated to induce caspase-8 mediated apoptosis. Fas also plays a role in T cell mediated cytotoxicity and T cell development. Fas is found in the thymus, liver, heart, and ovary, where it is expressed on a minority of resting T and B cells and monocytes. The antagonistic ZB4 clone anti-Fas monoclonal has been used to block the apoptosis-inducing activity of the Anti-Fas CH-11 clone in multiple publications.

This product does not induce apoptosis in cell culture.

특이성

This antibody does not recognize TNF and mouse Fas.

This antibody recognizes the human cell surface antigen Fas expressed in various human cells, including myeloid cells, T lympho-blastoid cells and diploid fibroblasts. It does not induce apoptosis in cell culture.

면역원

Recombinant human Fas.

애플리케이션

Anti-Fas Antibody (human, neutralizing), clone ZB4 detects level of Fas & has been published & validated for use in WB, FC, NEUT.

Flow Cytometry: A previous lot of this antibody used 5-20 μg/mL. Western Blot: A previous lot of this antibody used 5 μg/mL.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

품질

Evaluated by neutralization of apoptosis induced by anti-Fas (human, activating), clone CH11 (Catalog # 05-201) treatment of human Jurkat cells.

Neutralization: 10-500 ng/mL of this antibody was incubated for one hour with human Fas-transfected cells and inhibited the anti-Fas (human, activating), clone CH11 (Catalog # 05-201). Cell viability was assessed using a WST-1 assay

표적 설명

45 kDa

물리적 형태

Ammonium sulfate and purified mouse monoclonal IgG1 in PBS containing 50% glycerol. Store at -20°C.

Format: Purified

Protein A purified

저장 및 안정성

Stable for 1 year at from date of receipt.

분석 메모

Control
Various human cells

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2