05-173

Anti-Calmodulin Antibody

Upstate^®, from mouse

• 동의어(들): calmodulin 1, calmodulin 1 (phosphorylase kinase, delta), phosphorylase kinase, delta subunit
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: monoclonal
• 종 반응성: pig, mouse, vertebrates, rat, human, bovine
• 제조업체/상표: Upstate^®
• 기술: immunoprecipitation (IP): suitable; western blot: suitable
• 동형: IgG1
• NCBI 수납 번호: NP_008819
• UniProt 수납 번호: P62158
• 배송 상태: dry ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... CALM3(808)

설명

일반 설명

Calmodulin (CaM) is a ubiquitous, calcium-binding protein that can bind to and regulate a multitude of different protein targets, thereby affecting many different cellular functions.
CaM mediates processes such as inflammation, metabolism, apoptosis, muscle contraction, intracellular movement, short-term and long-term memory, nerve growth and the immune response. CaM is expressed in many cell types and can have different subcellular locations, including the cytoplasm, within organelles, or associated with the plasma or organelle membranes. Many of the proteins that CaM binds are unable to bind calcium themselves, and as such use CaM as a calcium sensor and signal transducer. CaM undergoes a conformational change upon binding to calcium, which enables it to bind to specific proteins for a specific response. CaM can bind up to four calcium ions, and can undergo post-translational modifications, such as phosphorylation, acetylation, methylation and proteolytic cleavage, each of which can potentially modulate its actions.

특이성

Also reacts to wheat germ. Wide cross-reactivity expected.

Specific for both Ca2+-bound and Ca2+-free calmodulin, Mr 17 kDa. Does not recognize bovine S100-alpha, S100-beta, troponin C or parvalbumin.

면역원

Epitope: a.a. 128-148

Synthetic peptide corresponding to the 21 carboxy-terminal amino acids (128-148) of bovine calmodulin, conjugated with tyroglobulin.

애플리케이션

Detect Calmodulin using this Anti-Calmodulin Antibody validated for use in WB & IP.

Immunoprecipitation:
4 μg of a previous lot immunoprecipitated calmodulin from A431 RIPA cell lysate. Radioimmunoassay:
Use 10 μg/mL to detect calmodulin in the range of 200-300 ng.

Research Category
Signaling

Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling

품질

Evaluated on human A431 carcinoma cells, RIPA lysates from mouse 3T3 and L6 rat skeletal fibroblasts.

Western Blot Analysis: 0.1-1 μg/mL of this antibody detected calmodulin in RIPA lysates from human A431 carcinoma cells. Previous lots detected calmodulin in RIPA lysates from mouse 3T3 and L6 rat skeletal fibroblasts.

표적 설명

17 kDa

결합

Replaces: 04-1158

물리적 형태

Format: Purified

Protein G Chromatography

Purified mouse IgG1 in storage buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide. Frozen solution.

저장 및 안정성

Stable for 1 year at from date of receipt.

분석 메모

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1