05-1320-M

Anti-EED Antibody, clone AA19

ascites fluid, from mouse

• 동의어(들): WD protein associating with integrin cytoplasmic tails 1, embryonic ectoderm development
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• 클론: monoclonal
• application: WB
• 종 반응성: human, mouse
• 기술: western blot: suitable
• citations: 8

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: ascites fluid
• 항체 생산 유형: primary antibodies
• 클론: monoclonal
• 종 반응성: human, mouse
• 기술: western blot: suitable
• 동형: IgG2aκ
• NCBI 수납 번호: NP_068676
• UniProt 수납 번호: O75530
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... EED(8726) ; mouse ... Eed(13626)

설명

일반 설명

Polycomb group proteins are important for maintaining transcriptional silencing. One conserved PcG complex, PRC2, is composed of several proteins including the histone methyltransferase EZH2, the WD-repeat protein EED (Embryonic ectoderm development), and the Zn-finger protein Suz12. Transcriptional repression mediated by EED involves histone deacetylation, while the EZH2 methylates histone H3 on lysine 27. EED protein is present in four isoforms. These EED isoforms selectively associate with distinct EZH2-containing complexes, resulting in differential targeting of their associated methyltransferase activity. These complexes play a role in Hox gene silencing, X-inactivation, germline development, stem cell pluripotency and cancer metastasis. EED is also known to bind H3K27me3 and can allosterically stimulate the methyltransferase activity of the PRC2 complex. (Ref.: Hojfeldt, JW. et al (2018) Nat. Struc. Mol. Biol. 25 (3); 225-232).

특이성

Not tested in other species.

This antibody recognizes mouse EED, (~30-65 kDa).

면역원

Recombinant fusion protein of full length murine EED tagged with MBP.

애플리케이션

Anti-EED Antibody, clone AA19 is a mouse monoclonal antibody for detection of EED also known as WD protein associating with integrin cytoplasmic tails 1 ,embryonic ectoderm development & has been validated in WB.

Research Category
Epigenetics & Nuclear Function

Apoptosis & Cancer

Research Sub Category
Chromatin Biology

품질

Routinely evaluated by Western Blot on A431cel lysatel.

Western Blot Analysis: 1:5,000 of this lot detected EED on 15 μg/mL of A431 cell lysate.

표적 설명

Ranges from 30-65 kDa (5-6 isoforms of EED are detected).

물리적 형태

Protein G Purified

Purified immunoglobulin in 0.1 M Tris-Glycine (pH7.4) 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

분석 메모

Control
A431 Cell Lysate

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable