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Merck
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Key Documents

04-914

Sigma-Aldrich

Anti-Smad2/3 Antibody, clone C4T, rabbit monoclonal

culture supernatant, clone C4T, from rabbit

동의어(들):

Deletion target in pancreatic carcinoma 4, MAD, mothers against decapentaplegic homolog 4, MAD, mothers against decapentaplegic homolog 4 (Drosophila), Mothers against DPP homolog 4, SMAD 4, SMAD family member 4, SMAD, mothers against DPP homolog 4, SMAD

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

rabbit

Quality Level

항체 형태

culture supernatant

항체 생산 유형

primary antibodies

클론

C4T, monoclonal

종 반응성

mouse, rat, human

기술

western blot: suitable

NCBI 수납 번호

UniProt 수납 번호

배송 상태

dry ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... SMAD2(4087)

일반 설명

Smad proteins are regulators of transcription which transduce signals from TGFβ Receptors. Smad proteins homotrimerize, and when activated, two distinct homotrimers assemble into a heterosextamer. Smad proteins fall into three classes. The receptor-regulated Smad proteins, Smad 1, 2, 3, 5, and 9 couple to specific receptors and are phosphorylated by those receptors. Phosphorylated receptor-regulated Smad proteins then bind to a co-Smad, such as Smad4/DPC4, and the complex moves to the nucleus where it associates with FAST-1 to stimulate target gene transcription. A third class of Smad proteins is the inhibitory group of Smad 6, 7, 8. Smad proteins have Mad-homology domains 1 and 2 (MH1 and MH2). MH1 domains are involved in DNA binding, while MH2 domains function in homotrimerization, receptor interaction and phosphorylation. Smad 4 mutations are frequently found in cancer, all of which cluster to the MH1 and MH2 domains of the protein. Those in the MH2 domain affect the ability of the protein to homotrimerize. The phosphorylation of Smad proteins is the regulatory signal in their activation, and can be monitored by the use of phosphorylation state-specific antibodies.

특이성

Predicted to cross-react with mouse and rat based on conservation of immunogen sequence.
Recognizes Smad2, Mr 55-60 kDa and Smad3, Mr 50 kDa.

면역원

Epitope: a.a. 186-273
Fusion protein corresponding to amino acids 186-273 of human Smad2.

애플리케이션

Research Category
Signaling
Research Sub Category
Transcription Factors
Use Anti-Smad2/3 Antibody, clone C4T (Rabbit Monoclonal Antibody) validated in WB to detect Smad2/3 also known as Deletion target in pancreatic carcinoma 4, mothers against decapentaplegic homolog 4.

품질

Evaluated by Western Blot on L6 lysates.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Smad2/3 in L6 cell lysate.

표적 설명

~50-60 kDa

결합

Replaces: 05-914

물리적 형태

Cultured supernantant containing 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

분석 메모

Control
RIPA lysates from L6 cells

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서 라이브러리 방문

Catherine Koch et al.
Molecular therapy. Methods & clinical development, 17, 1178-1189 (2020-06-10)
Myotubular myopathy, also called X-linked centronuclear myopathy (XL-CNM), is a severe congenital disease targeted for therapeutic trials. To date, biomarkers to monitor disease progression and therapy efficacy are lacking. The Mtm1-/y mouse is a faithful model for XL-CNM, due to

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