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Merck
모든 사진(1)

주요 문서

906581

Sigma-Aldrich

DLAM-AβVproS-13CH3 Methyl Labeling Kit

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About This Item

UNSPSC 코드:
12352200
NACRES:
NA.12

기술

bio NMR: suitable

Quality Level

배송 상태

dry ice

저장 온도

−70°C

관련 카테고리

일반 설명

DLAM-AβVproS-13CH3 kit has 13CH3 isotopomer precursors and contains protocol instructions for creation of isotopically-labeled proteins.

애플리케이션

DLAM-AβVproS-13CH3 is an optimized kit to produce proteins with 13CH3 isotopomer on β-methyl alanine and valine residues. This kit has been tested with protein isotopic labeling in E. coli. It is used in 1H-13C methyl TROSY approach to study the interactions and dynamics in very large protein complexes. For protein-protein complexes spectral complexity is reduced using differentially labeled samples.
For detection of long-range nOes or for the NMR study of large proteins

포장

This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.

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문서 라이브러리 방문

A new labeling method for methyl transverse relaxation-optimized spectroscopy NMR spectra of alanine residues.
Rivka L Isaacson et al.
Journal of the American Chemical Society, 129(50), 15428-15429 (2007-11-29)
Jose Luis Ortega-Roldan et al.
Methods in molecular biology (Clifton, N.J.), 1764, 73-85 (2018-04-02)
In this chapter, we describe how NMR chemical shift titrations can be used to study the interaction between two proteins with emphasis on mapping the interface of the complex and determining the binding affinity from a quantitative analysis of the
Silke Wiesner et al.
Current opinion in structural biology, 35, 60-67 (2015-09-26)
Intermolecular interactions are indispensible for biological function. Here we discuss how novel NMR techniques can provide unique insights into the assembly, dynamics and regulation of biomolecular complexes. We focus on applications that exploit the methyl TROSY effect and show that

문서

Methyl groups are ideal probes for studying protein structure and function by NMR. Choose among user-friendly kits for isotope labeling of a single type or multiple types of methyl groups.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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