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341738

Sigma-Aldrich

Sulforhodamine B, acid form

laser grade, Dye content 95 %

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About This Item

실험식(Hill 표기법):
C27H30N2O7S2
CAS Number:
2609-88-3
Molecular Weight:
558.67
EC Number:
220-025-2
MDL number:
MFCD00067690
UNSPSC 코드:
12352103
PubChem Substance ID:
57649121
NACRES:
NA.23

Grade

laser grade

형태

solid

구성

Dye content, 95%

mp

>300 °C (lit.)

λmax

558 nm

SMILES string

CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4S([O-])(=O)=O)S(O)(=O)=O)=[N+](/CC)CC)c1

InChI

1S/C27H30N2O7S2/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35)

InChI key

IOOMXAQUNPWDLL-UHFFFAOYSA-N

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일반 설명

Sulforhodamine B (SRB) is a fluorescent protein dye. It was reported that the dye binds itself to the amino acid residues of tricholoroacetic acid fixed cells in mild basic conditions and extracts itself into the solution under mild acidic conditions.

애플리케이션

pH sensitive of SRB towards it reactivity to living cells renders it useful for scaling drug induced cytotoxicity and cell proliferations. SRB assay was used to monitor the enzymatic activity of living cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)

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시험 성적서(COA)

Lot/Batch Number
MKCL1013
20223EA
05605DC
04920EQ
11917BU

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문서 라이브러리 방문

Wieland Voigt
Methods in molecular medicine, 110, 39-48 (2005-05-20)
The sulforhodamine B (SRB) assay was developed by Skehan and colleagues to measure drug-induced cytotoxicity and cell proliferation for large-scale drug-screening applications. Its principle is based on the ability of the protein dye sulforhodamine B to bind electrostatically and pH
Efficacy of 5-FU or Oxaliplatin Monotherapy over Combination Therapy in Colorectal Cancer.
Toloudi M, et al.
Journal of Cancer Therapy, 6(4), 345-345 (2015)
Minerva Nájera-Martínez et al.
Inhalation toxicology, 24(11), 762-773 (2012-09-08)
Halomethanes (HMs) can be formed during the chlorination process to obtain drinking water. In liver cells, HMs had been shown to be mutagenic and carcinogenic; however, their bioactivation by CYP 2E1 and GSTT1 is required. Although inhalation is the most
E E Gurdal et al.
Drug research, 63(3), 121-128 (2013-03-01)
This study presents the synthesis of nineteen 1-(substitutedbenzoyl)-4-benzhydrylpiperazine and 1-[(substitutedphenyl)sulfonyl]-4-benzhydrylpiperazine derivatives. In vitro cytotoxic activities of the compounds were screened against hepatocellular (HUH-7), breast (MCF-7) and colorectal (HCT-116) cancer cell lines by sulphorhodamine B assay. Among the test compounds, benzamide
Fujun Han et al.
Cytometry. Part A : the journal of the International Society for Analytical Cytology, 81(6), 532-540 (2012-05-11)
Multiparametric image analysis is highly preferable in revealing complicated biological processes. The multiplexing capability is demanding and is limited by spectral overlap of fluorescent dyes and the number of fluorescent channels available in an imaging platform, especially when both nuclear
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