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G005711

LC/MS Analysis of Omeprazole and Metabolites in Plasma on Ascentis® Express RP Amide after Sample Prep using HybridSPE®-Phospholipid

application for HPLC, application for SPE

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About This Item

technique(s)

HPLC: suitable
solid phase extraction (SPE): suitable

test parameters

sample preparation: SPE (Solid Phase Extraction)
sample/matrix: rat plasma spiked with omeprazole, phenacetin and metabolites at 2 μg/mL
SPE well plate: HybridSPE-Phospholipid, 96-well plate, 15 mg/0.8 mL (575656-U)
sample addition: 100 μL plasma to each well, followed by 300 mL of 1% formic acid in acetonitrile
elution: apply vacuum
eluate post-treatment: eluate was collected, evaporated, and reconstituted in mobile phase.
column: Ascentis Express RP-Amide, 5 cm x 2.1 mm I.D., 2.7 μm particles (53911-U)
mobile phase: [A] water, 10 mM ammonium formate, pH 3.4; [B] methanol, 10 mM ammonium formate, pH 3.4; (65:35, A:B)
flow rate: 0.3 mL/min
column temp.: 35 °C
detector: ESI(+), m/z = 50-400
injection: 1 μL

suitability

application for HPLC
application for SPE

application(s)

clinical

Application

This application demonstrates the suitability of HybridSPE and the Ascentis Express RP Amide for the analysis of omeprazole metabolites.

Analysis Note

Sample was vorlexed and centrifuged for 2 minutes at 15000 rpm. Supemate was then passed through HybridSPE 96 well plate. Elutent was collected, evaporated and reconstituted in mobile phase.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
HybridSPE is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    5-Hydroxyomeprazole
    Phenacetin
    Omeprazole
    Omeprazole sulfone

SPE tube or plate

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analytical column

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standard

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