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G005661

LC/MS Analysis of Anesthetics on Ascentis® Express F5

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis Express F5, 10 cm x 4.6 mm I.D., 2.7 μm particles (53590-U)
mobile phase: [A] 10 mM ammonium acetate, pH 4.0 with acetic acid; [B] acetonitrile; (20:80, A:B)
flow rate: 1.0 mL/min
pressure: 2320 psi (160 bar)
column temp.: 35 °C
detector: MS, ESI(+), SIR, m/z 235, 236, 247
injection: 2 μL
sample: 10 μg/mL in methanol

suitability

application for HPLC

application(s)

pharmaceutical (small molecule)

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Lidocaine
    Mepivacaine hydrochloride
    Procainamide hydrochloride

analytical column

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Description
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