80023448
TSKgel® UP-SW3000 (2 µm) HPLC Columns
L x I.D. 30 cm × 4.6 mm, diol phase
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product name
TSKgel® UP-SW3000 HPLC Column, phase diol, L × I.D. 30 cm × 4.6 mm, 2 μm particle size
material
stainless steel
Agency
suitable for USP L20
suitable for USP L59
product line
TSKgel®
manufacturer/tradename
Tosoh 0023448
parameter
≤0.5 M salt concentration
0-30% aqueous soluble organic solvents
0.35 mL/min flow rate
10-30 °C temp. range
340 bar pressure
technique(s)
HPLC: suitable
L × I.D.
30 cm × 4.6 mm
matrix
silica particle platform
matrix active group
diol phase
particle size
2 μm
pore size
250 Å
pH range
2.5-7.5
separation technique
size exclusion (SEC)
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General description
Other Notes
Legal Information
antibody
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Articles
These columns are designed to be operated with a simple and well established method (sodium phosphate mobile phase, pH 6.8) and are packed for use in both HPLC and UHPLC systems.
Informative article on size exclusion chromatography columns for biomolecule separations. Using columns packed with sub-2 μm particles provides significant gains in efficiency over SEC columns packed with larger particles.
Protocols
Bispecific antibodies are a new class of biotherapeutics where one therapeutic agent can target two different antigens. This application note demonstrates a new size exclusion chromatographic method for characterizing these new biotherapeutics by mass spectrometry.
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