ZRB1269

Anti-MAG Antibody, clone 1I6 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1I6, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 69.07 kDa; observed mol wt ~100 kDa
• species reactivity: rat, human, mouse
• species reactivity (predicted by homology): bovine, monkey
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal
• Protein ID accession no.: NP_002352.1
• UniProt accession no.: P20916
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 1I6 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Myelin-associated glycoprotein (MAG). It targets an epitope within 17 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human Myelin-associated glycoprotein (MAG).

Application

Quality Control Testing

Evaluated by Western Blotting in Rat brain membrane preparations.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Myelin-associated glycoprotein (MAG) in Rat brain membrane preparations.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Myelin-associated glycoprotein (MAG) in Human brain and Mouse brain microsomes.

Affinity Binding Assay: A representative lot of this antibody bound Myelin-associated glycoprotein (MAG) peptide with a KD of 1.0 x 10-12 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Myelin-associated glycoprotein (MAG) in human cerebellum tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-MAG, clone 1I6 ZooMAb^®, Cat. No. ZRB1269, is a recombinant Rabbit monoclonal antibody that targets Myelin-associated glycoprotein (MAG) and is tested for use in Affinity Binding Assay, Immunohistochemistry (Paraffin), and Western Blotting.

Target description

Myelin-associated glycoprotein (UniProt: P20916; also known as Siglec-4a, MAG) is encoded by the MAG (also known as GMA) gene (Gene ID: 4099) in human. MAG is a single-pass type I membrane protein that is expressed in myelin-forming cells when oligodendrites initiate contact with axons. It is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 20-516), a transmembrane domain (aa 517-536), and a cytoplasmic domain (aa 518-626). MAG is a member of the immunoglobulin superfamily that is found in both peripheral and CNS myelin, where it plays a role in the formation and maintenance of myelin sheath. It is not required for initial myelination, but seems to play a role in the maintenance of normal axon myelination. MAG is reported to protect motoneurons against apoptosis and after injury. Protection against apoptosis is probably mediated via interaction with neuronal reticulon receptor 4R (RTN4R) and Reticulon-4 receptor-like 2 protein (RTN4RL2). It is also considered to be important in maintenance of myelin with aging. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Poltorak, M., et al. (1987). J Cell Biol. 105(4); 1893-1899; Quarles, RH. (2007). J. Neurochem. 100(6); 1431-1448).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1