Z0627

Monoclonal Anti-ZAP-70 antibody produced in mouse

~1.5 mg/mL, clone 1E7.2, purified immunoglobulin, buffered aqueous solution

• MDL number: MFCD00803577
• UNSPSC Code: 12352203
• NACRES: NA.44

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 1E7.2, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~70 kDa
• species reactivity: human, mouse
• packaging: antibody small pack of 25 μL
• concentration: ~1.5 mg/mL
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 0.5-1.0 μg/mL using Jurkat cell extract
• isotype: IgG1
• UniProt accession no.: P43403
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ZAP70(7535) ; mouse ... Zap70(22637)

Description

General description

Monoclonal Anti-ZAP-70 (mouse IgG1 isotype) is derived from the hybridoma 1E7.2 produced by the fusion of mouse myeloma cells and splenocytes from mice. ZAP-70 is a protein tyrosine kinase of the Syk family that is localized exclusively to the cytosol of T cells and natural killer cells.

Immunogen

The immunogen sequence is conjugated to KLH.

peptide corresponding to amino acids 282-307 of human ZAP-70.

Application

Monoclonal Anti-ZAP-70 antibody has been used in

• enzyme linked immunosorbent assay (ELISA)
• immunoblottin
• flow cytometry
• immunoprecipitation
• immunocytochemistry

Monoclonal Anti-ZAP-70 antibody is suitable for use in western blot (0.5-1.0 μg/mL using Jurkat cell extract), flow cytometry, immunocytochemistry, immunoprecipitation, microarray and indirect ELISA.

Biochem/physiol Actions

ZAP-70 is a protein tyrosine kinase that is expressed in natural killer cells and T-lymphocytes. ZAP-70 interacts with the zeta chain of the T cell antigen receptor (TCR) and subsequently modulates cell signaling . Lack of ZAP-70 leads to an absence of antigen-specific response and also causes impaired T cell differentiation . Anti-ZAP-70 antibody is specific for human and mouse ZAP-70 (approx. 70 kDa).

Zeta-chain-associated protein kinase70 (ZAP-70) is required for T-cell activation and is activated by(Tyrosine protein kinase) Lck-mediated phosphorylation of its tyrosine residues. Mutation of the gene that creates a defective ZAP-70 results in severe combined immunodeficiency syndrome (SCID).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves