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Key Documents

SRP6382

Sigma-Aldrich

CD304/NRP1/Neurophilin-1 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Synonym(s):

NRP, VEGF165R

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in HEK 293 cells

tag

6-His tagged (C-terminus)

Assay

≥95% (SDS-PAGE)

form

lyophilized

mol wt

calculated mol wt 70.7 kDa
observed mol wt 85-90 kDa (DTT-reduced. Protein migrates due to glycosylation. Phe 22 is the predicted N-terminus.)

packaging

pkg of 10 μg

UniProt accession no.

O14786

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... NRP1(8829)

General description

Neuropilin-1 (NRP1) is also known as vascular endothelial cell growth factor 165 receptor (VEGF165R), CD antigen CD304, which belongs to the neuropilin family. The NRP1 gene is located on human chromosome 10p11.22.

Biochem/physiol Actions

Neuropilin-1 (NRP1) is membrane spanning protein that serves as coreceptor for a number of growth factors such as vascular endothelial growth factor, transforming growth factor β1, hepatocyte growth factor and platelet-derived growth factor. It is known to mediate immune responses, wound healing and angiogenesis, epithelial to mesenchymal transition in cancer. Mutation in the NRP1 gene results in tetralogy of Fallot (congenital heart disease).
The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, tThe PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis. The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The NRP1 migraine risk variant shows evidence of association with menstrual migraine.
Pollock C E, et al.
The Journal of Headache and Pain, 19(1), 31-31 (2018)
The Expression of neuropilin-1 in human placentas from normal and preeclamptic pregnancies.
Arad A, et al.
International Journal of Gynecological Pathology, 36(1), 42-49 (2017)
Neuropilin-1 is a receptor for extracellular miRNA and AGO2/miRNA complexes and mediates the internalization of miRNAs that modulate cell function.
Prud'homme G J, et al.
Oncotarget, 7(42), 68057-68057 (2016)
Functional polymorphisms of the neuropilin 1 gene are associated with the risk of tetralogy of Fallot in a Chinese Han population.
Fan S H, et al.
Gene, 653, 72-79 (2018)
L1 on ovarian carcinoma cells is a binding partner for Neuropilin-1 on mesothelial cells.
Stoeck A, et al.
Cancer Letters, 239(2), 212-226 (2006)

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