SAB4600116

Monoclonal Anti-GFP IgG (H+L), CF^™ 594 antibody produced in mouse

~2 mg/mL, clone 9F9.F9, purified immunoglobulin

• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: mouse
• conjugate: CF^™ 594 conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 9F9.F9, monoclonal
• form: buffered aqueous solution
• concentration: ~2 mg/mL
• technique(s): indirect immunofluorescence: 1-10 μg/mL
• isotype: IgG1κ
• fluorescence: λ_ex 593 nm; λ_em 614 nm
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Immunoglobulin G (IgG) belongs to immunoglobulin family and consists of gamma (γ) heavy chain in the constant (C) region. IgG is a widely expressed serum antibody. Monomeric structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa respectively, the primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, andγ4, respectively.1 IgG is produced and released from CD5⁺B cells, the predominant lymphocytes in the neonatal cell repertoire. Maternal IgG is the only antibody transported across the placenta to the fetus. Maternal IgG passively immunizes the infants.
CF^™ 594 is a deep red fluorescent dye with molecular weight ~730. CF^™ 594 is highly water soluble and photostable, therefore, it is considered to have a potential application in confocal microscopy and single molecule imaging. CF^™ 594 is particularly useful in combination with blue fluorescent CF^™ 350, green fluorescent CF^™ 488A and far red CF^™ 640R for multi-color imaging. CF^™ 594 has excitation laser line at 532nm, 568nm or 594nm and absorption/emission maxima at 593/614nm.
Green fluorescent protein (GFP) is highly visible and efficiently emitting internal fluorophore. These key features of GFP have made it a potential marker of gene expression and protein targeting in intact cells and organisms.

Specificity

Binds fluorescein

Immunogen

recombinant GFP

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Supplied in phosphate buffered saline with 0.05% sodium azide, 50% glycerol and 2 mg/mL bovine serum albumin.

Preparation Note

Protect from light.

Legal Information

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.

CF is a trademark of Biotium, Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable