Skip to Content
Merck
All Photos(2)

Key Documents

P3288

Sigma-Aldrich

Phosphate buffer pH 7.2 at 25 °C

for hematology and histology staining techniques

Synonym(s):

phosphate buffer

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352107
NACRES:
NA.47

Quality Level

form

powder

color

white

pH

7.10-7.30

application(s)

hematology
histology

storage temp.

room temp

General description

Phosphate buffer is the most commonly used component of irrigation solution during surgeries, to preserve the living tissues. It is also used in immunochemical staining procedures. Phosphate buffer serves as an important fixative for light and electron microscopy. Its buffering capacity is pH sensitive and is preferred to be used at physiological pH (7.2-7.4).

Application

Intended for use as a buffer in various Romanowsky type staining procedures, including Wright Stain, Wright Giemsa, Giemsa, May Grunwald, Jenner and Leishman. Phosphate buffer pH 7.2 at 25 °C has been used in oocyte collection from porcine ovaries.

Reconstitution

Each vial will prepare 3.8 L of buffer, 8.3 mM.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Biological Electron Microscopy: Theory, Techniques, and Troubleshooting (2011)
Low concentrations of MEM vitamins during in vitro maturation of porcine oocytes improves subsequent parthenogenetic development
Naruse K, et al.
Theriogenology, 67(2), 407-412 (2007)
Thaise L Teixeira et al.
Microbial pathogenesis, 135, 103618-103618 (2019-07-17)
P21 is a protein secreted by Trypanosoma cruzi (T. cruzi). Previous studies have shown a spectrum of biological activities performed by P21 such as induction of phagocytosis, leukocyte chemotaxis and inhibition of angiogenesis. However, the activity of P21 in T.

Related Content

Learn about the clinical study of blood, blood-forming organs, and blood diseases including the treatment, prevention, and stains and dyes used in hematology testing.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service