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N3786

Sigma-Aldrich

α(2→3,6,8,9) Neuraminidase from Arthrobacter ureafaciens

Proteomics Grade, suitable for MALDI-TOF MS

Synonym(s):

Neuraminidase from Arthrobacter ureafaciens, Acyl-neuraminyl Hydrolase, Sialidase

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About This Item

CAS Number:
9001-67-6
Enzyme Commission number:
3.2.1.18 (BRENDA, IUBMB)
EC Number:
232-624-6
MDL number:
MFCD00131711
UNSPSC Code:
12352204
NACRES:
NA.32

form

lyophilized powder

Quality Level

200

quality

Proteomics Grade

specific activity

≥25 U/vial

suitability

suitable for MALDI-TOF MS

storage temp.

2-8°C

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Application

Neuraminidase is an important deglycosylation enzyme capable of cleaving all non-reducing unbranched N-acetylneuraminic and N-glycolylneuraminic acid residues by hydrolysis of α(2→6), α(2→3), α(2→8), and α(2→9) linkages (affinity in the order given). Branched sialic acids may also be cleaved with the use of high concentrations of enzyme and prolonged incubations. Desialylated glycoproteins may then be further characterized by treatment with various exoglycosidases resulting in partial or complete O-deglycosylation. SDS-PAGE and MALDI-TOF MS are typically utilized in purification, structural analysis, and sequencing process. These techniques also remove heterogeneity and charge from the glycoprotein.

Packaging

Provided with 5× concentrate reaction buffer.

Quality

The buffer solution has been specifically formulated to assure maximum enzyme stability and assay sensitivity.

Unit Definition

One unit will release 1 nmole of 4-methylumbelliferone from 2-(4-methylumbelliferyl) α-D-N-acetylneuraminic acid per minute at pH 5.5 at 37° C.

Physical form

Lyophilized powder

Kit Components Also Available Separately

Product No.
Description
SDS
  • N3536Neuraminidase 5X Reaction Buffer 1.5 mLSDS

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Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SLCJ9368
SLCF3516
SLCC3203
SLBZ8459
SLBW3826

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Learn about O-linked glycan strategies, such as the actions of O-glycosidase, how to remove di and trisialylation sialic acid residues, β-linked galactose, and N-acetylglucosamine, as well as other O-glycan modifications.

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