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MAK120

Sigma-Aldrich

Urease Activity Assay Kit

sufficient for 100 colorimetric tests

Synonym(s):

Urea Amidohydrolase Activity Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

application(s)

environmental

detection method

colorimetric

relevant disease(s)

gastrointestinal diseases

storage temp.

2-8°C

Related Categories

General description

Urease is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Urease is found in bacteria, yeast, and certain higher plant species. Urease activity is found in environmental and fecal samples.

Application

Urease Activity Assay Kit has been used to measure urease activity.

Features and Benefits

Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.

Suitability

Suitable for the detection of Urease activity in biological and environmental samples.

Principle

In this assay, urease catalyzes the hydrolysis of urea resulting in the production of ammonia. The ammonia is determined by the Berthelot method resulting in colorimetric product measure at 670 nm, proportionate to the urease activity present in the sample. One unit of urease is the amount of enzyme that catalyzes the formation of 1.0 μmole ammonia per minute at pH 7.0.

Signal Word

Danger

Hazard Statements

H290,H314,H341,H410

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1B

Supplementary Hazards

EUH031

Storage Class Code

8B - Non-combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Isolation, partial purification, characterization and inhibition of urease (EC 3.5. 1.5) enzyme from the Cajanus cajan seeds.
Sujoy B and Aggarwal A
Asian Journal of Bio Science, 7(2), 203-209 (2012)
Joudeh B Freij et al.
Infection and immunity, 86(7) (2018-05-02)
The genus Cryptococcus includes several species pathogenic for humans. Until recently, the two major pathogenic species were recognized to be Cryptococcus neoformans and Cryptococcus gattii We compared the interaction of murine macrophages with three C. gattii species complex strains (WM179
Short-term treatment with the urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT) alters urea assimilation and modulates transcriptional profiles of genes involved in primary and secondary metabolism in maize seedlings.
Zanin L, et al.
Frontiers in Plant Science, 7, 845-845 (2016)
Chelsea M Heveran et al.
Scientific reports, 9(1), 14721-14721 (2019-10-13)
We demonstrate for the first time that the morphology and nanomechanical properties of calcium carbonate (CaCO3) can be tailored by modulating the precipitation kinetics of ureolytic microorganisms through genetic engineering. Many engineering applications employ microorganisms to produce CaCO3. However, control
Ureases I. Functional, catalytic and kinetic properties: A review
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Journal of Molecular Catalysis. B, Enzymatic, 59(1-3), 9-21 (2009)
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