L3397
LB Broth (Luria low salt)
Powder microbial growth medium
Synonym(s):
Luria Broth (Miller’s Modification), Luria-Bertani medium (LB)
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About This Item
UNSPSC Code:
41106200
NACRES:
NA.85
Recommended Products
grade
for molecular biology
Quality Level
sterility
non-sterile
form
powder
composition
NaCl, 0.5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L
technique(s)
microbiological culture: suitable
pH
6.8-7.2(1.55% solution)
suitability
suitable for molecular biology
nonselective for Escherichia coli
nonselective for coliforms
application(s)
food and beverages
microbiology
storage temp.
room temp
General description
Luria Broth is a bacterial culture medium used for the cultivation of members of the Enterobacteriaceae especially E.coli, as well as for coliphage plaque assays. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a very low-salt formulation.
Application
LB Broth (Luria low salt) has been used as a liquid broth medium in the broth microdilution technique.
Features and Benefits
Luria′s low salt LB powder provides:
- Standard formulation
- A budget-friendly alternative to liquid
- Easy scale-up using larger package sizes
Preparation Note
1. Suspend 15.5 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.
2. Autoclave for 15 minutes at 121°C.
Reconstitution
Stir to suspend 15.5g powder in 1L water. Autoclave for 15 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
related product
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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