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GE28-9365-49

HiPrep™ Heparin Fast Flow 16/10

Cytiva 28-9365-49

Synonym(s):

Heparin affinity chromatography

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About This Item

UNSPSC Code:
13111000
NACRES:
NA.56

ligand

porcine heparin

form

prepacked column

crosslinking

cross-linked

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 28-9365-49

storage condition

(20% ethanol containing 0.05 M sodium acetate)

parameter

1.5 bar (22 psi) (Over the Packed Bed During Operation)
2 ml/min - 10 ml/min flow rate
22 psi

technique(s)

liquid chromatography (LC): suitable

bed size

16 mm × 100 mm

bed volume

20 mL

column I.D.

16 mm

matrix

6% cross-linked agarose

matrix active group

heparin

particle size

45-165 μm

average diameter

90 μm

cleaning

4-13

working range

4-12

suitability

suitable for bioprocess medium

separation technique

affinity

General description

HiPrep™ Heparin FF 16/10 is prepacked with Heparin Sepharose™ 6 Fast FLow for preparative purification of proteins with affinity for heparin such as DNA-binding proteins, coagulation factors, lipoproteins, and protein synthesis factors.

Application

The column provides fast, preparative separations of Proteins and other biomolecules. Immobilized heparin has two main modes of interaction with Proteins. It can operate as an affinity ligand with affinity for a wide range of biomolecules, including coagulation factors and other plasma Proteins, lipoProteins, Protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors. Heparin also functions as a high-capacity cation exchanger, due to its anionic sulfate groups. Gradient elution with salt is most commonly used in both cases. Heparin is coupled to Sepharose™ 6 Fast FLow with a chemically optimized linkage.

Heparin is a mucopolysaccharide consisting of a repeating dimer of α-L-dipyranuronic acid 2-sulfate, and 2-deoxy-2-sulfamino-α-D-glucopyranose 6-sulfate, with a molecular weight range of 5 000 to 30 000.

Features and Benefits

  • 20 mL column optimized for convenient scale-up purification .
  • Immobilized heparin is used for purification of antithrombin III, coagulation factors, and other plasma Proteins.
  • Simple operation with single pump configurations, as well as ÄKTA® design systems.
  • Excellent flow characteristics.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.05 M Sodium Acetate containing 20% Ethanol)
Store at 4 to 30 °C (20% Ethanol, 0.05 M Sodium Acetate)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiPrep is a trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

related product

Product No.
Description
Pricing

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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