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HCT-15

91030712, human colon, Epithelial-like

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About This Item

UNSPSC Code:
41106514

Product Name

HCT-15, 91030712

biological source

human colon

growth mode

Adherent

karyotype

2n = 46, quasidiploid

morphology

Epithelial-like

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human colon adenocarcinoma

Cell Line Description

Colorectal adenocarcinoma which is tumorigenic in nude mice.The four cell lines: DLD-1 (90102540), HCT-15 (91030712), HCT-8 (90032006) and HRT-18 (86040306) have been shown to have a common genetic origin.

Application

HCT-15 cell line has been used to study the role and efficiency of nexrutine (NX), an extract from Phellodendron amurense, against colon cancer.

Culture Medium

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS) (heat inactivated).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

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Articles

ECACC Nucleic Acids

DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.

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