Ehrlich-Lettre Ascites strain E cell line

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: mouse unknown/unspecified
• packaging: tube of 5 μg 87032503-DNA-5UG; pkg of vial of cells 87032503-1VL
• growth mode: Adherent
• karyotype: 2n = 40
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse carcinoma

Cell Line Description

Established from the explant of a 7 day old tumour from the parental Ehrlich-Lettre Ascites carcinoma. Prior to development, strain E was subjected to a schedule of seven mouse passages and six in vitro passages over a period of 17 months. The cells exhibit an unusually high mean chromosome number and possess the marker chromosomes present in the parental Ehrlich tumour. This cell line may be of use in comparative testing of anti-tumour agents in vivo and in vitro.

Application

Anti-tumour test and tumourigenicity studies

Culture Medium

NCTC 135 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm²using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Media change 3 times a week.

Other Notes

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