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Key Documents

LLCMK2 (Original)

from rhesus monkey kidney, 85062804, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)

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About This Item

UNSPSC Code:
41106514

biological source

rhesus monkey kidney

growth mode

Adherent

karyotype

2n = 42

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Monkey Rhesus kidney

Cell Line Description

Susceptible to a wide range of viruses including enterovirus, rhinovirus, myxovirus and poxvirus groups.

Application

LLCMK2 (Original) has been used to infect and maintain Trypanosoma cruzi parasite culture and as a medium for Sendai virus titer determination. It has also been used for viral culture.

Culture Medium

EMEM (EBSS) (M2279) + 2 mM L-Glutamine (G7513) + 1% NEAA (M7145) + 5% FBS / FCS (F2442).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37 °C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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