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C6366

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EX-CELL® CHO Cloning Medium

Animal-component free, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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About This Item

UNSPSC Code:
12352207

Quality Level

400

sterility

sterile-filtered

product line

EX-CELL®

form

liquid

technique(s)

cell culture | mammalian: suitable (suspension)

components

L-glutamine: no
NaHCO3: yes

shipped in

ambient

storage temp.

2-8°C

Related Categories

General description

Developed to meet the needs of the biotechnology industry, this animal component-free medium is designed for single-cell cloning of recombinant CHO cell lines adapted to serum-free suspension culture.

Application

This animal component-free medium is designed to support clonal survival and growth of Chinese Hamster Ovary (CHO) cell lines, with results comparable to the traditional method using 10% fetal bovine serum. Aseptically add 10 mL of 200 mM L-glutamine solution per 500 mL of medium prior to use.

Legal Information

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Using Microarray Technology to Select Housekeeping Genes in Chinese Hamster Ovary Cells

In the present study, we have identifi ed species-specifi c housekeeping genes (HKGs) for Chinese Hamster Ovary (CHO) cells using data from microarray gene expression profiling.

Selection Process Development in a GS Knock-Out CHO Host Cell Line: The Effects of MSX Addition on Clones Generated in an MSX-Free Process
Mgat4 May Play a Role in Increased Sialylation by Overexpressing Functional MGAT1 in Mgat1-Disrupted Chinese Hamster Ovary (CHO) Cells

MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells.

Signal Peptide Optimization: Effect On Recombinant Monoclonal IgG Productivity, Product Quality And Antigen-Binding Affinity

A signal peptide is a 5-30 amino acid (aa) peptide present at the N-terminus of secretory proteins.

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