C3138

Cathepsin D from bovine spleen

lyophilized powder, ≥2.0 units/mg protein

• CAS Number: 9025-26-7
• Enzyme Commission number: 3.4.23.5 (BRENDA, IUBMB)
• MDL number: MFCD00130749
• UNSPSC Code: 12352204
• NACRES: NA.32

Properties

• form: lyophilized powder
• Quality Level: 200
• specific activity: ≥2.0 units/mg protein
• mol wt: ~45 kDa
• UniProt accession no.: P80209
• storage temp.: −20°C
• Gene Information: cow ... CTSD(282883)

Description

Biochem/physiol Actions

Cathepsin D is an endosomal-lysosomal aspartic protease implicated in breast cancer metastasis and Alzheimer′s disease. Lysosomal release of cathepsin D has been found to precede cytochrome c release and loss of membrane potential in apoptotic human foreskin fibroblasts. Cathepsin D levels in PC12 cells increase 12 to 24 hours after apoptosis is induced.

Unit Definition

One unit will produce an increase in A₂₈₀ of 1.0 per min per mL at pH 3.0 at 37 °C measured as TCA-soluble products using hemoglobin as substrate (1 cm light path).

Physical form

Lyophilized powder containing citrate buffer salts

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable