C1057

Butyrylcholinesterase from equine serum

lyophilized powder, ≥900 units/mg protein

• Synonym(s): Acylcholine acyl-hydrolase, Choline esterase, butyryl, Pseudocholinesterase
• CAS Number: 9001-08-5
• Enzyme Commission number: 3.1.1.8 (BRENDA, IUBMB)
• EC Number: 232-579-2
• MDL number: MFCD00081455
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• form: lyophilized powder
• specific activity: ≥900 units/mg protein
• mol wt: tetramer 440 kDa
• composition: Protein, ≥10%
• solubility: H₂O: soluble 10 mg/mL
• application(s): diagnostic assay manufacturing
• storage temp.: −20°C

Description

Application

Selective inhibition of BChE activity can be used in the detection of organophosphates. Its use in the treatment of organophosphate toxicity has been explored. It has been reported that the level of BChE in human blood correlates to the degree of protection against potentially toxic nerve agents. Cholinesterases have also been investigated for their role in Alzheimer′s disease.

Biochem/physiol Actions

Butyrylcholinesterase (BChE) is a serine hydrolase that is structurally similar to acetylcholinesterase (AChE), but differs in substrate specificities and inhibitor sensitivities.BChE can, unlike AChE, efficiently hydrolyze larger esters of choline such as butyrylcholine and benzoylcholine. The enzyme is a tetrameric glycoprotein with four equal subunits (110 kDa each). The enzyme is activated by Ca²⁺ and Mg²⁺. The activity is constant over the pH range of 6.0-8.0. It is inhibited by betaine, nicotine, organophosphates, and carbamate.

Unit Definition

One unit will hydrolyze 1.0 μmole of butyrylcholine to choline and butyrate per min at pH 8.0 at 37 °C. The activity obtained using butyrylcholine as substrate is ~2.5 times that obtained using acetylcholine.

Physical form

Highly purified; contains buffer salts

Analysis Note

Protein determined by biuret

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves