C0543

Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane

Type IV (Miller), lyophilized powder, BioReagent, suitable for cell culture

• Synonym(s): Type IV collagen, EHS-collagen
• CAS Number: 9007-34-5
• EC Number: 232-697-4
• MDL number: MFCD00130825
• UNSPSC Code: 12352202
• NACRES: NA.75

Properties

• biological source: mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane)
• Quality Level: 200
• type: Type IV (Miller)
• product line: BioReagent
• form: lyophilized powder
• packaging: glass bottle of 1 vial
• technique(s): cell culture | mammalian: suitable
• surface coverage: 6‑10 μg/cm²
• solubility: 0.25% acetic acid: 0.5-2.0 mg/mL (Keep at 2-8 °C for several hours, occasionally swirling.)
• UniProt accession no.: B1AVK5; P02463; P08122; Q63ZW6; Q9QZS0
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: mouse ... Col4a1(12826) , Col4a2(12827) , Col4a3(12828) , Col4a5(12830) , Col4a6(94216)

Description

General description

Collagen is classified into several structurally and genetically distinct types. Type IV collagen is a predominant structural protein of the basement membrane. It is notably characteristic of the Engelbreth-Holm-Swarm (EHS) mouse sarcoma. It is commercially available as matrigel and cultrex. Matrigel is made of type 4 collagen, laminin, heparan sulfate proteoglycan and entactin obtained from engelbreth-holm-swarm murine sarcoma. The target cells for attachment include: epithelial cells, endothelial cells, muscle cells, and nerve cells.

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Application

Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane has been used:

• in adhesion assays.
• in hydrogel fabrication.
• to coat cell culture plates for cell differentiation.
• to treat round plastic coverslips for scanning electron microscopy (SEM) analysis in an interaction study between A. culbertsoni trophozoites and Schwann cells.

Recommended for use as a cell culture substratum at 6-10 μg/cm².

Biochem/physiol Actions

Collagen isolated from Engelbreth-Holm-Swarm murine sarcoma basement membrane helps in the development of 3D nodules. It is also used in 2D coatings.

Other Notes

Native collagen

We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma-type designations with recognized collagen classification types.

Quantity

0.75 mg/vial

Preparation Note

Chloroform treated.
Pepsin is not used in the preparation.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves