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Key Documents

B4561

Sigma-Aldrich

Anti-53BP1 (N-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-TP53BP1, Anti-p53 Binding Protein 1

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About This Item

MDL number:
MFCD09039189
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 345 kDa

species reactivity

mouse (predicted), rat (predicted), human

concentration

~1 mg/mL

technique(s)

immunoprecipitation (IP): 4-8 μg using HEK 293-T cell lysates
microarray: suitable
western blot: 0.5-1 μg/mL using U2OS total cell extracts

UniProt accession no.

Q12888

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TP53BP1(7158)
mouse ... Trp53bp1(27223)
rat ... Tp53bp1(296099)

Specificity

Anti-53BP1 (N-terminal) specifically recognizes 53BP1.

Immunogen

synthetic peptide corresponding to amino acids 23-41 of human 53BP1. The immunizing peptide sequence differs from that of mouse and rat by two and three amino acids, respectively.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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