A9294
Anti-Goat IgG (whole molecule)−Agarose antibody produced in rabbit
IgG fraction of antiserum, PBS suspension
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About This Item
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biological source
rabbit
Quality Level
conjugate
agarose conjugate
antibody form
IgG fraction of antiserum
antibody product type
secondary antibodies
clone
polyclonal
form
PBS suspension
species reactivity
goat
extent of labeling
~2.4 mg Ab per mL
technique(s)
immunoelectrophoresis: suitable
matrix
Cross-linked 4% beaded agarose
matrix activation
cyanogen bromide
matrix spacer
1 atom
capacity
1 mg/mL, resin binding capacity (goat IgG)
storage temp.
2-8°C
target post-translational modification
unmodified
General description
An immunoglobulin has two heavy chains and two light chains connected by disulfide bond. It mainly helps in immune defense. . IgG, a glycoprotein, is the major class of immunoglobulin. Goat IgG has two subclasses- IgG1 and IgG2.
IgGs are glycoprotein antibodies that modulate several immune responses. Goat IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-goat IgGs are useful tools for the analysis of target proteins.
Specificity
Rabbit Anti-Goat IgG (whole molecule)-Agarose antibody binds to goat IgG.
Immunogen
pooled normal goat serum
Application
Anti-Goat IgG (whole molecule) Agarose antibody produced in rabbit has been used in protein purification, immunoprecipitation/chromatin immunoprecipitation assay.
c-RET was immunoprecipitated from overexpressing MDCK cells using agarose-linked rabbit anti-goat IgG. Homogenates were incubated with conjugated beads for 2 hours on ice.
Biochem/physiol Actions
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.
Physical form
Suspension in phosphate buffered saline containing 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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