A7921
Agar
suitable for plant cell culture, powder
Synonym(s):
Agar-agar, Gum agar
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All Photos(4)
About This Item
Linear Formula:
(C12H18O9)n
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
50405901
eCl@ss:
42040102
NACRES:
NA.21
Recommended Products
biological source
algae (Rhodophyceae)
Quality Level
form
powder
technique(s)
cell culture | plant: suitable
transition temp
congealing temperature 30-35 °C
application(s)
agriculture
storage temp.
room temp
InChI
1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1
InChI key
GYYDPBCUIJTIBM-DYOGSRDZSA-N
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General description
Agar contains agarose and agaropectin, with agarose being the major gelling heteropolysaccharide. Agarose contains β-d-galactose and 3,6-anhydro-α-l-galactose, linked by glycosidic bonds β(1-4) called the neoagarobiose. It also has agarobiose with same disaccharide units with α(1-3) linkage. The major source of agar is the cell wall of seaweed from Rhodophyceae family.
Application
Agar has been used as a component of EDMm (modified version of Eucalyptus dunnii basal medium (EDM)) for development of shoots of Eucalyptus dunnii clones. It has also been used as a component of Murashige and Skoog medium for in vitro culture of Strain TC09 of Cladosporium sphaerospermum.
Agar has been used as a component
- of Gamborg′s B5 regeneration medium
- of Murashige and skoog (MS) medium for the germination of Hypericum triquetrifolium Turra seeds
- in shoot inducing and naphthalene acetic acid pretreatment medium for the regeneration of Arabidopsis thaliana seedlings
Biochem/physiol Actions
Agar is majorly used as microbial solid culture media. It is used in the range of 0.5 to 1.0% (w/v) to solidify plant tissue culture media. It is essential for in vitro root development.
Other Notes
Purified for research and protoplast culture.
Preparation Note
Typical working concentration: 6-12 g/L.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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