A6279
Aprotinin from bovine lung
saline solution, 3-7 TIU/mg protein
Synonym(s):
BPTI, Bovine pancreatic trypsin inhibitor, Trasylol, Trypsin inhibitor (basic)
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About This Item
Empirical Formula (Hill Notation):
C284H432N84O79S7
CAS Number:
Molecular Weight:
6511.44
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.77
Recommended Products
biological source
bovine
Quality Level
sterility
aseptically filled
form
saline solution
specific activity
3-7 TIU/mg protein
mol wt
~6,500
solubility
H2O: soluble >10 mg/mL
UniProt accession no.
storage temp.
2-8°C
InChI key
ZPNFWUPYTFPOJU-UHFFFAOYSA-N
Gene Information
cow ... PTI(404172)
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General description
Aprotinin is a 58 amino acid-long protein, with a calculated molecular weight of 6512 Da, that acts as a serine protease inhibitor. Known also as bovine pancreatic trypsin inhibitor (BPTI), aprotinin inhibits serine proteases such as chymotrypsin, kallikrein, plasmin and trypsin.
Application
Aprotinin is largely used as an inhibitor of trypsin.
Biochem/physiol Actions
Aprotinin is a competitive serine protease inhibitor that forms stable complexes with and blocks the active sites of enzyme. This binding is reversible, and most aprotinin-protease complexes will dissociate at extreme pH levels >10 or <3. Structurally, Aprotinin is a monomeric globular protein derived from bovine lung that consists of 58 amino acids, arranged in a single polypeptide chain with three crosslinking disulfide bridges.
Caution
Aprotinin products should be stored at 2-8°C.
Unit Definition
One Trypsin Inhibitor Unit (TIU) will decrease the activity of two trypsin units by 50%, where one trypsin unit will hydrolyze 1.0 μmole of N-alpha-benzoyl-DL-arginine p-nitroanilide per minute at pH 7.8 and 25°C. Another commonly used unit is the KIU, with 1 TIU = 1,300 KIU.
Preparation Note
This product is an aseptically filled solution in 0.9% NaCl and 0.9% benzyl alcohol. It has an activity of 3-7 TIU/mg protein and 3-21 TIU/mL solution.
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Protocols
Enzymatic Assay of Aprotinin
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