79454
Ampliflu™ Red Western Blot Kit
for fluorescent detection
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About This Item
UNSPSC Code:
12352200
Recommended Products
usage
sufficient for 50 applications
fluorescence
λex 571 nm; λem 585 nm
storage temp.
2-8°C
General description
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Application
The Ampliflu Red Western Blot Kit provides an alternative to the classical chemiluminescence detection. When working with Ampliflu Red, standard protocols for the immunoblotting-procedure can be used until the detection step. No extra time for signal accumulation is necessary as it is for chemiluminescent signals. The use of Ampliflu Red is suitable for protein quantities between 1 ng and 1 μg per band of protein.
Principle
The kit contains the peroxidase substrate Ampliflu Red that is enzymatically converted to the highly fluorescent compound, resorufin. Fluorescent imaging is then performed on the immunoblot.
Analysis Note
Sensitivity: Limit of detection is 1 ng/band, but may vary depending on the antibodies used.
Legal Information
Ampliflu is a trademark of Sigma-Aldrich Co. LLC
Kit Components Only
Product No.
Description
- Dimethyl sulfoxide, puriss. p.a., ACS reagent, ≥99.9% (GC) 3 mL
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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