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52526

Sigma-Aldrich

Anti-Human IgG (whole molecule)−Atto 488 antibody produced in goat

~1 mg/mL

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

conjugate

Atto 488 conjugate

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

contains

50% glycerol as stabilizer

species reactivity

human

concentration

~1 mg/mL

fluorescence

λex 500 nm; λem 522 nm in PBS

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders,. Atto 488-goat anti-human IgGs are known to associate with human IgGs.

Immunogen

human IgG

Application

Atto 488-goat anti-human IgG may be used for fluorescence-based immunoassays.

Physical form

Atto 488 goat anti-human IgG (whole molecule) is provided in unit sizes of 1 ml as 1 mg/ml solutions in 0.1 M sodium phosphate, 0.1 M NaCl, pH 7.5, containing 5 mM sodium azide as a preservative.

Analysis Note

unconjugated dye ≤5% of total fluorescence; dye-to protein ration ≥2

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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W Wisemandle et al.
Pediatrics, 106(1), E14-E14 (2000-07-06)
To determine the effects of timing of onset of overweight (OW) on childhood anthropometric adiposity levels using long-term serial data from the Fels Longitudinal Study. OW was defined as body mass index (BMI; kg/m(2)) >25, and the age at first
Breaking the Diffraction Barrier in Fluorescence Microscopy by Optical Shelving.
Bretschneider, S.; Eggeling, Chr.; Hell, S. W.
Physical Review Letters, 98(21) (2007)
F Cataldo et al.
Minerva pediatrica, 42(12), 509-514 (1990-12-01)
IgG are the most common isotype of Ig and include four subclasses which differ from one another in the following ways: their initial amino acid sequence, their physical and chemical properties and the different serum concentrations reached with age. Every
C Papadea et al.
Critical reviews in clinical laboratory sciences, 27(1), 27-58 (1989-01-01)
Human IgG consists of two identical heavy (H) chains and two identical light (L) chains joined by interchain disulfide bridges. Heterogeneity in the amino acid sequences of the H and L polypeptides results in at least three types of IgG
Dual color localization microscopy of cellular nanostructures.
Gunkel, M.; et al.
Biotechnology Journal, 4(6), 927-938 (2009)
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