50649

Glyoxal solution

for molecular biology, BioReagent, ~40% in H₂O (~8.8 M)

• Synonym(s): Ethanedial, Oxalaldehyde
• Linear Formula: OHCCHO
• CAS Number: 107-22-2
• Molecular Weight: 58.04
• Beilstein: 1732463
• MDL number: MFCD00006957
• UNSPSC Code: 12352114
• PubChem Substance ID: 57651264
• NACRES: NA.52

Properties

• grade: for molecular biology
• product line: BioReagent
• form: liquid
• concentration: ~40% in H₂O (~8.8 M)
• impurities: DNases, none detected; RNases, none detected; phosphatases, none detected; proteases, none detected
• refractive index: n20/D 1.409
• anion traces: chloride (Cl^-): ≤100 mg/kg; sulfate (SO₄^2-): ≤100 mg/kg
• cation traces: Ca: ≤5 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Na: ≤300 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Zn: ≤5 mg/kg
• storage temp.: 2-8°C
• SMILES string: [H]C(=O)C([H])=O
• InChI: 1S/C2H2O2/c3-1-2-4/h1-2H
• InChI key: LEQAOMBKQFMDFZ-UHFFFAOYSA-N

Description

General description

Glyoxal is a nucleic acid denaturant. It acts as a specific probe for homopurine strands of DNA.

Application

Suitable for

• nucleic acid denaturation
• binding of DNA/RNA to nitrocellulose membrane

Other Notes

This form of glyoxal is composed of 3 moles of glyoxal and 2 moles of water in a relatively stable configuration.

May precipitate on storage; redissolve at 50-60 °C.

Caution

May precipitate on storage; redissolve at 50-60 °C.

Principle

Glyoxal forms stable complex with guanine residues that results in denaturation of nucleic acids.

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Warning
• Hazard Statements: H315,H317,H319,H332,H335,H341
• Precautionary Statements: P202 - P280 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Inhalation - Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves