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P5504

Sigma-Aldrich

Potassium phosphate dibasic trihydrate

ReagentPlus®, ≥99.0%

Synonym(s):

Dipotassium hydrogen phosphate trihydrate, Dipotassium phosphate, di-Potassium hydrogen phosphate trihydrate

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About This Item

Linear Formula:
K2HPO4 · 3H2O
CAS Number:
16788-57-1
Molecular Weight:
228.22
EC Number:
231-834-5
MDL number:
MFCD00149926
UNSPSC Code:
12161700
PubChem Substance ID:
57654537
NACRES:
NA.25

Quality Level

200

product line

ReagentPlus®

Assay

≥99.0%

form

powder or crystals

pH

9.2-9.4 (25 °C, 50 g/L)

solubility

water: 100 mg/mL, clear, colorless

storage temp.

room temp

SMILES string

O.O.O.[K+].[K+].OP([O-])([O-])=O

InChI

1S/2K.H3O4P.3H2O/c;;1-5(2,3)4;;;/h;;(H3,1,2,3,4);3*1H2/q2*+1;;;;/p-2

InChI key

XQGPKZUNMMFTAL-UHFFFAOYSA-L

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General description

Potassium phosphate solution have very high buffering capacity that is widely used in molecular biology, biochemistry, and chromatography. Potassium phosphate dibasic trihydrate is a key component of potassium phosphate buffer, phosphate-buffered saline (PBS), and phosphate-buffered EDTA (PBE). It is extensively used in the preparation of biological samples for various biological experiments. Potassium phosphate buffer is also used in methods such as equilibrium dialysis, high-performance liquid chromatography (HPLC), and affinity capillary electrophoresis (ACE) to study the characteristics of drug-protein interactions in the body. Potassium phosphate buffer solutions consist of mixtures of the potassium phosphate monobasic and potassium phosphate dibasic forms.

Application

Potassium phosphate dibasic trihydrate has been used in the preparation of potassium phosphate buffer for the resuspension of polyelectrolyte capsules. It has also been used as a buffer during glutaminase‑1 (GLS-1) enzyme assay and GLS-2 enzyme assay.

Legal Information

ReagentPlus is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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