CRM12900L
Escherichia coli O157 (NT) NCTC 12900 LENTICULE® discs
30-120 CFU, suitable for microbiology, certified reference material, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Synonym(s):
Microbiological standards, Certified reference material, E. coli O157 (NT) NCTC 12900 Lenticule® discs
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About This Item
UNSPSC Code:
41116107
NACRES:
NA.24
grade
certified reference material
Quality Level
Agency
ISO 17034
ISO/IEC 17025
form
disc (hydrosoluble)
solid
shelf life
limited shelf life, expiry date on the label
packaging
package of 10 discs
manufacturer/tradename
Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
application(s)
environmental
food and beverages
microbiology
storage temp.
−20°C
suitability
Escherichia coli
coliforms
General description
Escherichia coli O157 (NT) NCTC 12900 Lenticule® discs are Certified Microorganisms Standards (Certified Microbiological Reference Materials), which can be used as quantitative and qualitative control organisms. They contain colony-forming units (CFUs) in the range of 30-120 per disc.
The technology allows long-term conservation and renders the organisms resistant to temperature changes. They are intended to be used as daily internal quality control organisms.
These microorganisms are certified according to ISO/IEC 17025 and produced under reproducible conditions compliant with ISO 17034 using authenticated strains from NCTC. These ready-to-use microbiological controls minimize the need for maintaining control strains in the test laboratory and guarantee the use of an authenticated control culture for every quality control test.
Lenticule® discs are prepared from a traceable culture obtained freeze-dried from the National Collection of Type Cultures (NCTC) or National Collection of Pathogenic Fungi (NCPF) and are manufactured by Sigma-Aldrich under license from UK Health Security Agency.
The technology allows long-term conservation and renders the organisms resistant to temperature changes. They are intended to be used as daily internal quality control organisms.
These microorganisms are certified according to ISO/IEC 17025 and produced under reproducible conditions compliant with ISO 17034 using authenticated strains from NCTC. These ready-to-use microbiological controls minimize the need for maintaining control strains in the test laboratory and guarantee the use of an authenticated control culture for every quality control test.
Lenticule® discs are prepared from a traceable culture obtained freeze-dried from the National Collection of Type Cultures (NCTC) or National Collection of Pathogenic Fungi (NCPF) and are manufactured by Sigma-Aldrich under license from UK Health Security Agency.
Application
Escherichia coli O157 (NT) NCTC 12900 Lenticule® discs are used as reference strains in routine quality control for food, beverage, water, and environmental testing. Additionally, they are excellent for use in method development and validations, as starter cultures, and for use in research studies and educational purposes.
Features and Benefits
- Ready-to-use at convenient CFU ranges
- No dilution required
- Quick rehydration in 10 minutes
- At least 16 months of shelf life in a standard -20 °C freezer
- Complete with a comprehensive certificate of analysis
- No maintenance of stock cultures
- No recovery time or pre-enrichment step is necessary
- Robust, efficient, and reliable
- Easy-to-use
- Cost-effective
Other Notes
Strain origin: NCTC 12900
Other cell culture collections:
Other cell culture collections:
- DSM 17076
- ATCC® 700728
- CECT 4972
Strain History - Accessioned into the NCTC in 1996 < Dr Henry R. Smith (CPHL, Public Health Laboratory Service, Colindale, London, UK) < Isolated by Federal Public Health Lab (Innsbruck, Austria), from unknown substrate, Innsbruck Austria, in 1992
Legal Information
ATCC is a registered trademark of American Type Culture Collection
Lenticule is a registered trademark of The Secretary of State for Health (United Kingdom)
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
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