B8806
2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester
1 mg/mL in DMSO
Synonym(s):
BCECF-AM solution, Spiro(isobenzofuran-1(3H),9′-(9H)xanthene)-2′,7′-dipropanoic acid
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About This Item
description
Mixture of six isomeric compounds
Quality Level
form
liquid
concentration
1 mg/mL in DMSO
color
colorless to yellow
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
−20°C
SMILES string
CC(=O)OCOC(=O)CCc1cc2c(Oc3cc(OCOC(C)=O)c(CCC(=O)OCOC(C)=O)cc3C24OC(=O)c5cc(ccc45)C(=O)OCOC(C)=O)cc1OCOC(C)=O
Application
2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester has been used for the estimation of vacuolar pH and intracellular pH.
Biochem/physiol Actions
An intracellular fluorescent pH indicator.
Hydrolyzed to BCECF by cytosolic esterases.
Physical form
Solution in 0.1% BCECF AM Ester and 99.9% DMSO
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
185.0 °F
Flash Point(C)
85 °C
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A K Ho et al.
The American journal of physiology, 261(4 Pt 1), C642-C649 (1991-10-01)
In rat pinealocytes, activation of alpha 1-adrenergic receptors leads to increases in intracellular pH (pHi). In this study, the role of pHi on adrenergic regulation of cyclic nucleotide accumulation was investigated using ammonium chloride, which increased pHi, and sodium propionate
Salil Chanroj et al.
The Journal of biological chemistry, 286(39), 33931-33941 (2011-07-29)
The complexity of intracellular compartments in eukaryotic cells evolved to provide distinct environments to regulate processes necessary for cell proliferation and survival. A large family of predicted cation/proton exchangers (CHX), represented by 28 genes in Arabidopsis thaliana, are associated with
Chengzhi Hu et al.
Lab on a chip, 17(4), 671-680 (2017-01-18)
Pollen tubes are tip-growing plant cells that deliver the sperm cells to the ovules for double fertilization of the egg cell and the endosperm. Various directional cues can trigger the reorientation of pollen tube growth direction on their passage through
Viraj P Nirwan et al.
Nanomaterials (Basel, Switzerland), 11(2) (2021-03-07)
Herein, we report the fabrication and characterization of novel polycaprolactone (PCL)-based nanofibers functionalized with bare (ligand-free) titanium nitride (TiN) nanoparticles (NPs) for tissue engineering applications. Nanofibers were prepared by a newly developed protocol based on the electrospinning of PCL solutions
Evaluation of intracellular pH regulation and alkalosis defense mechanisms in preimplantation embryos.
Dagilgan S, et al.
Theriogenology, 83, 1075-1084 (2015)
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