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83913

Sigma-Aldrich

RNA isolation kit

BioUltra, for molecular biology

Synonym(s):

Ribonucleic acid isolation kit

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.25

grade

for molecular biology

Quality Level

100

product line

BioUltra

storage temp.

2-8°C

Related Categories

General description

RNA isolation kit utilizes a rapid single-step extraction (guanidine thiocyanate/phenol/chloroform extraction) to isolate undegraded total RNA; the kit contains enough reagents to isolate total RNA from at least 7g of tissue.
Content: Denaturating solution, phenol saturated with H2O, β-mercaptoethanol, chloroform:isoamyl alcohol, 2 M sodium acetate, isopropanol, and instruction manual.

Other Notes

Kit used for the isolation of RNA

Signal Word

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Repr. 2 - Skin Corr. 1B - Skin Sens. 1 - STOT RE 1 Oral - STOT RE 2 - STOT RE 2 Oral - STOT SE 3

Target Organs

Central nervous system, Heart

Supplementary Hazards

EUH032

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

53.6 °F - closed cup

Flash Point(C)

12 °C - closed cup

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Certificates of Analysis (COA)

Lot/Batch Number
BCCJ5235
BCCF4478
BCCB2950
BCBX5100
BCBR1427V

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Michael J McKenzie et al.
Journal of sports science & medicine, 10(2), 385-392 (2011-01-01)
Genes can be activated or inhibited by signals within the tissues in response to an acute bout of exercise. It is unclear how a particular aerobic exercise bout may influence two muscles with similar actions to the activity. Therefore, the
J M Chirgwin et al.
Biochemistry, 18(24), 5294-5299 (1979-11-27)
Intact ribonucleic acid (RNA) has been prepared from tissues rich in ribonuclease such as the rat pancreas by efficient homogenization in a 4 M solution of the potent protein denaturant guanidinium thiocyanate plus 0.1 M 2-mercaptoethanol to break protein disulfide
Kuan-Bing Chen et al.
Cell death & disease, 12(8), 748-748 (2021-07-30)
Chemotherapy remains the primary treatment of advanced solid cancer, including lung cancer. However, as first-line treatment, cisplatin-based therapy is restricted by the frequent development of drug resistance. Increasing data showed that the programmed cell death protein ligand 1 (PD-L1) plays
P Chomczynski et al.
Analytical biochemistry, 162(1), 156-159 (1987-04-01)
A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is

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