70192
Mueller Hinton Broth
suitable for microbiology, NutriSelect® Plus
Synonym(s):
MH Broth; Antibacterial potency testing media; Antimicrobial susceptibility testing media
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About This Item
UNSPSC Code:
41171504
NACRES:
NA.74
Recommended Products
sterility
non-sterile
Quality Level
product line
BioChemika
form
powder
shelf life
limited shelf life, expiry date on the label
composition
beef infusion solids, 2.0 g/L
casein hydrolysate, 17.5 g/L
starch, 1.5 g/L
manufacturer/tradename
NutriSelect® Plus
storage condition
protect from light
technique(s)
microbe id | susceptibility testing: suitable
microbiological culture: suitable
final pH
7.4±0.2 (25 °C)
application(s)
clinical testing
food and beverages
microbiology
storage temp.
15-25°C
suitability
nonselective for bacteria (General Media)
Related Categories
General description
Mueller Hinton Broth is used for determining minimal inhibitory concentrations (MICs). Mueller Hinton medium is recommended by FDA, World Health Organization, and NCCLS for testing the most encountered aerobic and facultative anaerobic bacteria in food and clinical material. The medium shows good batch-to-batch reproducibility, it is low in sulfonamide, trimethoprim, and tetracycline inhibitors and yields satisfactory growth of most non-fastidious pathogens.
Application
Mueller Hinton broth is primarily used to determine the MIC (minimum inhibitory concentration) for several antimicrobial agents. It can also be used to cultivate fastidious organisms like Meningococcus and Gonococcus
Preparation Note
Dissolve 21 g in 1 litre of distilled water. Sterilize by autoclaving at 121°C for 15 minutes.
Footnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Branko Jovčić et al.
Antibiotics (Basel, Switzerland), 9(12) (2020-12-17)
The antimicrobial susceptibility testing was conducted on 174 single isolates from poultry farms in Serbia and it was determined that seven Salmonella spp. were multidrug resistant. Sixteen serotypes were detected, but only serotype Infantis confirmed reduced susceptibility to colistin. Seven
Konrad Gwozdzinski et al.
Journal of clinical microbiology, 56(5) (2018-03-09)
The plasmid-located colistin resistance gene mcr-1 confers low-level resistance to colistin, a last-line antibiotic against multidrug-resistant Gram-negative bacteria. Current CLSI-EUCAST recommendations require the use of a broth microdilution (BMD) method with cation-adjusted Mueller-Hinton (CA-MH) medium for colistin susceptibility testing, but
Pin-Kuang Lai et al.
Biotechnology and bioengineering, 116(10), 2439-2450 (2019-06-19)
Proline-rich antimicrobial peptides (PrAMPs) kill bacteria via a nonlytic mechanism in which they permeate through the outer membrane, utilize protein-mediated transport across the inner membrane, and target the ribosome to inhibit protein synthesis. We previously reported that substitutions of oncocin
J.H. Muller et al.
Proceedings of the Society For Experimental Biology and Medicine. Society For Experimental Biology and Medicine, 48, 330-330 (1941)
Tracy Lackraj et al.
Journal of bacteriology, 202(11) (2020-04-02)
Dps, a DNA-binding protein from starved cells in Escherichia coli, is part of the bacterial defense system that protects DNA against various cellular stresses. Our lab previously demonstrated that a novel antimicrobial peptide, WRWYCR, enhances acid-induced killing of enterohemorrhagic Escherichia
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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