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Key Documents

30911

Sigma-Aldrich

Denaturation solution for the isolation of RNA

BioUltra, for molecular biology

Synonym(s):

RNA isolation, denaturation

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About This Item

MDL number:
MFCD00217302
UNSPSC Code:
12352200
NACRES:
NA.25

grade

for molecular biology

Quality Level

100

product line

BioUltra

composition

guanidine thiocyanate, 4 M
Na-citrate, 25 mM
sarcosyl, 0.5%

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

refractive index

n20/D 1.433

pH

5.5-7.0

anion traces

chloride (Cl-): ≤500 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤2 mg/kg
As: ≤0.1 mg/kg
Ba: ≤2 mg/kg
Bi: ≤2 mg/kg
Ca: ≤10 mg/kg
Cd: ≤2 mg/kg
Co: ≤2 mg/kg
Cr: ≤2 mg/kg
Cu: ≤2 mg/kg
Fe: ≤2 mg/kg
K: ≤100 mg/kg
Li: ≤2 mg/kg
Mg: ≤2 mg/kg
Mn: ≤2 mg/kg
Mo: ≤2 mg/kg
Ni: ≤2 mg/kg
Pb: ≤2 mg/kg
Sr: ≤2 mg/kg
Zn: ≤2 mg/kg

λ

neat

UV absorption

λ: 280 nm Amax: 1

Application

Denaturation solution for the isolation of RNA is a guanidine thiocyanate, sarcosyl based protein denaturization solution useful for the purification of mRNA.

Other Notes

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

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Certificates of Analysis (COA)

Lot/Batch Number
BCCF1640
BCBS3081V
BCBK2156V
BCBH0592V
BCBG7029V

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Y L Tsai et al.
Applied and environmental microbiology, 57(3), 765-768 (1991-03-01)
A protocol for direct extraction of mRNA from soil samples was developed. Soil samples (10 g) were washed twice with 120 mM phosphate buffer (pH 5.2). The lysis of cells, fixation of RNA, and hydrolysis of DNA were achieved by
P Chomczynski et al.
Analytical biochemistry, 162(1), 156-159 (1987-04-01)
A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is

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