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244813

Sigma-Aldrich

Reactive Blue 4

Dye content 35 %

Synonym(s):

1-Amino-4-[3-(4,6-dichlorotriazin-2-ylamino)-4-sulfophenylamino]anthraquinone-2-sulfonic acid, Procion® blue MX-R

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About This Item

Empirical Formula (Hill Notation):
C23H14Cl2N6O8S2
CAS Number:
13324-20-4
Molecular Weight:
637.43
Colour Index Number:
61205
Beilstein:
602258
EC Number:
236-363-9
MDL number:
MFCD00001218
UNSPSC Code:
12171500
PubChem Substance ID:
24854708
NACRES:
NA.47

form

powder

Quality Level

200

composition

Dye content, 35%

λmax

595 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Nc1c(cc(Nc2ccc(c(Nc3nc(Cl)nc(Cl)n3)c2)S(O)(=O)=O)c4C(=O)c5ccccc5C(=O)c14)S(O)(=O)=O

InChI

1S/C23H14Cl2N6O8S2/c24-21-29-22(25)31-23(30-21)28-12-7-9(5-6-14(12)40(34,35)36)27-13-8-15(41(37,38)39)18(26)17-16(13)19(32)10-3-1-2-4-11(10)20(17)33/h1-8,27H,26H2,(H,34,35,36)(H,37,38,39)(H,28,29,30,31)

InChI key

RTLULCVBFCRQKI-UHFFFAOYSA-N

Gene Information

rat ... P2rx1(25505)

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General description

Reactive blue 4 (C.l. 61205) is a dichlorotriazine dye also known as procion brilliant blue MX-R, procion brilliant blue M.

Application

Reactive blue dyes have been used for labeling antibodies. Such anthraquinone dyes are widely used as immobilized ligands in systems for protein separations. Reactive blue 4 is used as a tracer to assess apical leakage in dental fillings. It is routinely used to stain proteins on electrophoretic gels.

Legal Information

Procion is a registered trademark of Dystar Textilfarben GmbH & Co.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Precautionary Statements

P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBN7078
MKCJ6381
MKCH5412
MKCG4689
MKCB3736

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A Durán et al.
Water research, 41(3), 690-698 (2006-10-03)
The heterogeneous photocatalytic degradation of reactive blue 4 dye (RB4) solutions under Fenton reagent and TiO(2) assisted by concentrated solar light irradiation using a Fresnel lens has been studied. Multivariate experimental design was applied to study the kinetic process. The
Ramida Yuwadee Watanapokasin et al.
Biodegradation, 20(3), 411-418 (2008-11-13)
Anaerobic biodegradability of wastewater (3,000 mg CODcr/l) containing 300 mg/l Reactive Blue 4, with different co-substrates, glucose, butyrate and propionate by a bacterial consortium of Salmonella subterranea and Paenibacillus polymyxa, concomitantly with hydrogen production was investigated at 35 degrees C.
Shwu-Jinng Wang et al.
Analytical biochemistry, 361(2), 190-196 (2007-01-02)
For investigating the feasibility of using reactive dyes as an immunoassay marker, a dichlorine triazine dye, Procion Blue MX-7RX, was employed to stain the antibody against human serum albumin (anti-HSA). With the color intensity revealed in the immunochromatographic test strip
A Boonyakamol et al.
Applied biochemistry and biotechnology, 158(1), 180-191 (2008-08-06)
The factors affecting decolorization of anthraquinone dye represented by Reactive Blue 4 (RB4) and azo dye represented by Methyl Orange (MO) were studied in batch experiments under mesophilic (35 degrees C) and thermophilic (55 degrees C) anaerobic conditions. The results
A Boonyakamol et al.
Applied biochemistry and biotechnology, 152(3), 405-417 (2008-06-11)
Anaerobic decolorization of anthraquinone dye represented by Reactive Blue 4 (RB4) was studied to evaluate the factors involved in dye-reducing behaviors such as dye concentration, co-substrate, treatment temperature, salt content, and dye-reducing microbial consortia. The experiment was conducted using digested
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