WBLUC
Immobilon Classico Western HRP substrate
Western blotting, dot/slot blotting
Synonym(s):
HRP substrate, chemiluminescent detection substrate, detection substrate
About This Item
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conjugate
peroxidase conjugate
Quality Level
manufacturer/tradename
Luminata™
technique(s)
western blot: suitable
membrane area
1000 cm2 , membrane coverage
detection method
chemiluminescent
shipped in
ambient
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A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.
Protocols
Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.
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