UFC205024

Amicon^® Ultra Centrifugal Filter, 50 kDa MWCO

sample volume 2 mL, regenerated cellulose membrane, MWCO 50 kDa

• Synonym(s): Centrifugal concentrator, 50 kDa MWCO, 2 mL sample volume
• UNSPSC Code: 41104916
• eCl@ss: 36100101
• NACRES: NB.24

Properties

• material: polypropylene tube (for collection); regenerated cellulose membrane; styrene-butadiene housing
• Quality Level: 400
• sterility: non-sterile
• product line: Amicon^®
• manufacturer/tradename: Amicon^® Ultra
• parameter: 2 mL sample volume
• technique(s): protein extraction: suitable; protein purification: suitable
• L: 119.7 mm
• diam.: 15.9 mm
• filtration area: 1 cm²
• pore size: 50 kDa MWCO
• shipped in: ambient

Description

General description

Amicon^® Ultra-2 mL centrifugal devices with Ultracel^® 50,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. For example, BSA (67 kDa) at 1 mg/ml initial concentration is typically retained at 97% .
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 50-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
The device can be spun in a swinging bucket or fixed angle rotor. Amicon^® Ultra-2 mL devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon^® Ultra 2 mL filters deliver the highest recovery with shortest spin times.

Application

• Ultrafiltration with 50,000 Dalton molecular weight cutoff Ultracel^® membrane.
• Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
• Purification of macromolecular components found in tissue culture extracts or cell lysates.
• Primer removal, PCR cleanup, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to HPLC.
• Desalting, buffer exchange and protein dialysis.
• Size exclusion and protein fractionation.

Features and Benefits

• Ultracel^® regenerated cellulose membrane with 50 kDa NMWCO delivers retentate recovery of greater than 90%
• Concentrates 2 mL down to 15-70 μL
• 98% sample recovery
• Fast processing time: 10 to 30-minute spin times
• Engineered dead stop
• Reverse spin capability provides consistent recoveries

Linkage

Replaces: 4224

Physical form

Color Code: Clear

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany

ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany